Journal: Frontiers in Neurology
Article Title: Characterization of a humanized mouse model of Charcot-Marie-Tooth type 1A for the discovery of human PMP22 -targeting drugs
doi: 10.3389/fneur.2025.1658204
Figure Lengend Snippet: Quantitative analysis of plasma NfL levels in humanized mice and PMP22-C3 mice. Plasma NfL levels were measured using the SiMoA immunoassay in WT, (A) homo-humanized, (B) hetero-humanized, and (C) PMP22-C3 mice at 9, 14, and 18 weeks of age. Data are represented as mean ± SEM ( n = 8–10/group). Statistical comparisons were performed using two-way Repeated Measures ANOVA with a post hoc Bonferroni’s test ( ** p < 0.01, *** p < 0.001 vs. WT mice). WT, wild-type; NfL, neurofilament light chain; SiMoA, single molecule array; SEM, standard error of the mean.
Article Snippet: NfL levels in plasma were measured using an HD-X analyzer (Quanterix Corporation, Massachusetts, USA) with a Simoa NF-LightTM V2 Advantage Kit (104,073, Quanterix) according to the manufacturer’s instructions.
Techniques: Clinical Proteomics